Page 13 - Cleanroom_Genano

Nanoscale Particle Purification

Particle removal efficiency, VTT 2017
GENANO PURIFICATION EFFICIENCY AS A FUNCTION OF PARTICLE SIZE

100

Particle removal efficiency (%) 80 Very high purification efficiency
even for nanosied particles.

60

40 HEPA PM 2,5
Ultrafine particles < 100 nm 0,3 μm

20

0

0,001 0,01 0,1 1 10

Particle size (µm) DATA FROM ORIGINAL REPORT OF VTT, FINLAND, 2017
*Measurement of Genano 5250 Air Cleaner with ultrafine particles*

TEST SETUP

• DNA fragments produced by PCR
• Length 264 base pairs (90 nm)

• Nebulised with a Laskin-type aerosol generator
• Diluted with purified air

• Filtration efficiency
• Determined by comparing DNA concentrations downstream of the air decontamina-
tion unit when purification on / off
• Nuclepore 0.4 µm sample filters, qPCR analysis of filters, stirred in 4 ml of TE buffer

Test High Stream Volume Sample DNA concentration E
No voltage of air dm3 suspension (molecules/microliter)
volume ml 99,97
1 m3 1 2 Mean 99,71
2 4 8,2E+00 1,9E+00 5,1E+00
3 on 80 426 (air) 4 8,3E+01 7,0E+01 7,7E+01 99,95
4 on 150 785 (air) 4 2,5E+04 2,9E+04 2,7E+04 99,99
5 off 150 796 (air) 4 2,0E+04 1,8E+04 1,9E+04
6 off 80 404 (air) 4 1,0E+01 7,1E+00 8,6E+00
7 on 80 447 (air) 1,7E+02 1,5E+02 1,6E+02
8 * 2 (water) 4 0,0E+00 3,6E+00 1,8E+00
on 80 401 (air) 5,1E+02 5,6E+02 5,4E+02
* 1 (water)

*The washing liquid was collected after the run of the tests no 1–5 (test 6) and after the test no 7 (test 8).